data/YM_0549/README.md

VHH72 Optimization Variants Iter0 x CoV2-RBD (YM_0549)

Overview

YM_0549 consists of VHH72 designs against SARS CoV-2 RBD. We wanted to perturb the local landscape of VHH72, so we performed mutagenesis to observe the sensitivity of VHH-72 to different RBD targets. This dataset is great to study the local sensitivity of mutations relative to parent "WT" VHH72.

Experimental details

This dataset includes 29765 unique VHHs and 8 unique RBD sequences. The alpha-library is the same as YM_1068.

A more extensive methods section can be found in our publication here.

Misc dataset details

We define the following binders:

A-library (scFvs)

There are several terms you can filter by:

• wt_<i>: These are WT replicates.
• candidate_: Various mutations of VHH-72

Alpha-library

• SARS-CoV2_RBD_(6LZG)
• WIV1
• LYRa11
• MERS_RBD
• OMICRON_BA2_TStarr_Seq&Trunc
• SARS-COV-2_GAMMA_RBD_LLNL_Trunc
• SARS-CoV-2_Delta_RBD_L452R_T478K
• SARS-CoV1_RBD_(6LZG_Mimic)