# VHH72 Optimization Variants Iter0 x CoV2-RBD (YM_0549)

## Overview

YM_0549 consists of VHH72 designs against SARS CoV-2 RBD. We wanted to perturb the local landscape of VHH72, so we performed mutagenesis to observe the sensitivity of VHH-72 to different RBD targets. This dataset is great to study the local sensitivity of mutations relative to parent "WT" VHH72.

## Experimental details

This dataset includes 29765 unique VHHs and 8 unique RBD sequences. The alpha-library is the same as YM_1068.

A more extensive methods section can be found in our publication [here](https://pmc.ncbi.nlm.nih.gov/articles/PMC12296056/).

## Misc dataset details

We define the following binders:

### A-library (scFvs)

There are several terms you can filter by:

- `wt_<i>`: These are WT replicates.
- `candidate_`: Various mutations of VHH-72

### Alpha-library

- `SARS-CoV2_RBD_(6LZG)`
- `WIV1`
- `LYRa11`
- `MERS_RBD`
- `OMICRON_BA2_TStarr_Seq&Trunc`
- `SARS-COV-2_GAMMA_RBD_LLNL_Trunc`
- `SARS-CoV-2_Delta_RBD_L452R_T478K`
- `SARS-CoV1_RBD_(6LZG_Mimic)`