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  1. .gitattributes +1 -0
  2. FIELDS_DESCRIPTION.md +74 -0
  3. README.md +4 -2
  4. Stimuli/DS_setup1.pdf +0 -0
  5. Stimuli/DS_setup3.pdf +0 -0
  6. Stimuli/RGC_MovingBar_setup1.pdf +0 -0
  7. Stimuli/RGC_MovingBar_setup3.pdf +0 -0
  8. Stimuli/chirp1000_setup1.pdf +0 -0
  9. Stimuli/chirp1000_setup3.pdf +0 -0
  10. Stimuli/chirp800_setup1.pdf +0 -0
  11. Stimuli/chirp800_setup3.pdf +0 -0
  12. Stimuli/processed_stimuli.h5 +2 -2
  13. production_export.log +0 -0
  14. session_Experimenter_1_2022-05-31/experiment_1_LR/field_GCL0.nwb +2 -2
  15. session_Experimenter_1_2022-05-31/experiment_1_LR/field_GCL0_structure.txt +8 -2
  16. session_Experimenter_1_2022-05-31/experiment_2_RR/field_GCL1.nwb +2 -2
  17. session_Experimenter_1_2022-05-31/experiment_2_RR/field_GCL1_structure.txt +8 -2
  18. session_Experimenter_1_2022-06-10/experiment_1_LR/field_GCL1.nwb +2 -2
  19. session_Experimenter_1_2022-06-10/experiment_1_LR/field_GCL1_structure.txt +8 -2
  20. session_Experimenter_1_2022-06-10/experiment_2_RR/field_GCL0.nwb +2 -2
  21. session_Experimenter_1_2022-06-10/experiment_2_RR/field_GCL0_structure.txt +8 -2
  22. session_Experimenter_1_2022-06-14/experiment_1_LR/field_GCL1.nwb +2 -2
  23. session_Experimenter_1_2022-06-14/experiment_1_LR/field_GCL1_structure.txt +8 -2
  24. session_Experimenter_1_2022-07-07/experiment_1_LR/field_GCL1.nwb +2 -2
  25. session_Experimenter_1_2022-07-07/experiment_1_LR/field_GCL1_structure.txt +8 -2
  26. session_Experimenter_1_2022-07-12/experiment_2_RR/field_GCL2.nwb +2 -2
  27. session_Experimenter_1_2022-07-12/experiment_2_RR/field_GCL2_structure.txt +8 -2
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  29. session_Experimenter_1_2022-07-14/experiment_2_RR/field_GCL0_structure.txt +8 -2
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  31. session_Experimenter_1_2022-07-28/experiment_2_RR/field_GCL2_structure.txt +8 -2
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  41. session_Experimenter_1_2022-08-16/experiment_1_LR/field_GCL0_structure.txt +8 -2
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  49. session_Experimenter_1_2022-08-16/experiment_2_RR/field_GCL1_structure.txt +8 -2
  50. session_Experimenter_1_2022-08-16/experiment_2_RR/field_GCL2.nwb +2 -2
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+ *.nwb filter=lfs diff=lfs merge=lfs -text
FIELDS_DESCRIPTION.md ADDED
@@ -0,0 +1,74 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ # Field Descriptions for `all_GCL_table.parquet` and `.nwb` files
2
+
3
+ This document describes the data fields available in the `all_GCL_table.parquet` file and corresponding `.nwb` files.
4
+
5
+ ## General Metadata
6
+
7
+ - **experimenter**: Name of the experimenter who conducted the recording
8
+ - **date**: Date of the experiment
9
+ - **exp_num**: Experiment number for the given date. Typically corresponds to the eye: 1 = left eye, 2 = right eye
10
+ - **field**: Field identifier in the experiment. Format: "GCL" or "RGC" followed by a number. The number indicates the sequential order of the field within the recording session. Field numbering conventions may vary across experimenters (typically starting at 0 or 1). Some fields may include postfixes (e.g., "a" or "b") to indicate additional experimental conditions or retinal subdivisions
11
+ - **region**: Anatomical region of the recording field. Values: "LR" (Left Retina), "RR" (Right Retina), "RRN" (Right Retina Nasal), "RRT" (Right Retina Temporal)
12
+ - **roi_id**: Region of interest identifier within the field
13
+ - **cond1**: Condition identifier. Values: "C1", "c1", or "control", indicating the first control condition. Some experiments include multiple control or drug conditions
14
+ - **stim_name**: Name of the stimulus protocol used
15
+ - **genline**: Genetic line of the animal (typically "Bl6")
16
+ - **animgender**: Sex of the animal. Values: "female", "male", or "" (empty string indicates missing data)
17
+ - **age**: Age of the animal in days at the time of the experiment
18
+ - **eye**: Eye used for recording. Values: "left" or "right"
19
+ - **prepwmorient**: Orientation of the eye in degrees. 0° indicates dorsal is toward the back of the chamber. -1 indicates unknown orientation
20
+ - **roi_size_um2**: Area of the region of interest mask in square micrometers
21
+ - **ventral_dorsal_pos_um** (and **field_ventral_dorsal_pos_um**): Ventral-dorsal position relative to the optic nerve in micrometers. Negative values indicate ventral location; positive values indicate dorsal location
22
+ - **temporal_nasal_pos_um** (and **field_temporal_nasal_pos_um**): Temporal-nasal position relative to the optic nerve in micrometers. Negative values indicate temporal location; positive values indicate nasal location
23
+
24
+ ## Cell Type Classification
25
+
26
+ - **Celltype**: Functional cell type classification according to the classifier
27
+ - **Max_confidence**: Confidence score for the cell type classification (0-1 scale)
28
+ - **preproc_chirp**: Preprocessed chirp average response, resampled and clipped to match the format used in Baden et al. (2016)
29
+ - **preproc_bar**: Preprocessed moving bar response, shifted to match the format used in Baden et al. (2016)
30
+
31
+ ## Chirp Stimulus Responses
32
+
33
+ - **chirp_on_off_index** (OOi): On-Off index from chirp stimulus. Values: +1 = On cell, -1 = Off cell, 0 = On-Off cell or non-responsive
34
+ - **chirp_transience_index** (RTi): Response transience index. Values: +1 = very transient response, 0 = very sustained response. Negative values are not well defined
35
+ - **chirp_qidx**: Quality index (signal-to-noise ratio) for the chirp response
36
+ - **chirp_pres_qidx**: Percentage of cells in the field that pass the quality threshold (0.35)
37
+ - **chirp_us_average_norm** or **chirp_60Hz_average_norm**: Resampled, normalized chirp average response at 60 Hz sampling rate
38
+ - **chirp_us_average_dt** or **chirp_60Hz_average_dt**: Sampling interval of the upsampled chirp average (1/60 seconds)
39
+ - **chirp_8Hz_average_norm**: Resampled, normalized chirp average response at approximately 8 Hz (7.8125 Hz). Similar to `preproc_chirp` but without reshaping to match Baden et al. (2016) format
40
+ - **chirp_8Hz_average_dt**: Sampling interval of the 8 Hz chirp average (1/7.8125 seconds)
41
+ - **chirp_snippets**: Chirp responses organized by repetition/trial
42
+ - **chirp_snippets_t0**: Starting time point of each repetition
43
+ - **chirp_snippets_dt**: Sampling interval of the snippets (0.128 seconds)
44
+ - **chirp_triggertimes_snippets**: Trigger times for chirp snippets, required for precise temporal alignment
45
+
46
+ ## Moving Bar Stimulus Responses
47
+
48
+ - **bar_qidx**: Quality index for moving bar response (calculated using only the preferred direction)
49
+ - **bar_ds_index** (DSi): Direction selectivity index
50
+ - **bar_ds_pvalue**: Statistical p-value for direction selectivity
51
+ - **bar_pref_dir**: Preferred direction in radians
52
+ - **bar_os_index** (OSi): Orientation selectivity index
53
+ - **bar_os_pvalue**: Statistical p-value for orientation selectivity
54
+ - **bar_pref_or**: Preferred orientation in radians. Note: This value is not corrected for eye orientation (left vs. right) or experimental setup geometry
55
+ - **bar_avg_pref_dir**: Average preferred direction response at 7.81 Hz sampling rate
56
+
57
+ ## Receptive Fields
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+
59
+ The following fields are available when the experiment included white noise stimuli:
60
+
61
+ - **rf_cdia_um**: Circle-equivalent diameter of the spatial receptive field in micrometers
62
+ - **srf**: Spatial receptive field
63
+ - **trf**: Temporal receptive field
64
+ - **center_index**: Strength and sign of the RF center. Values: +1 = ON center, -1 = OFF center
65
+ - **surround_index**: Strength and sign of the RF surround. Values: +1 = ON surround, -1 = OFF surround. Note: For meaningful analysis, the sign of the center must be taken into account
66
+ - **split_qidx**: Quality index of the singular value decomposition (SVD) used to separate spatial and temporal components from the spatiotemporal RF
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+ - **rf_gauss_qidx**: Quality index of the Gaussian fit to the spatial RF
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+ - **polarity**: Overall polarity of the receptive field. Values: +1 = ON, -1 = OFF
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+ - **trf_peak_idxs**: Indices of peaks in the temporal RF
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+ - **relx_rf_roi_um**: Offset in setup coordinates between the ROI and RF center along the x-axis (back to front)
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+ - **rely_rf_roi_um**: Offset in setup coordinates between the ROI and RF center along the y-axis (left to right)
72
+ - **tRF_tRI**: Transience index calculated from the temporal RF
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+ - **tRF_width**: Width of the main peak in the temporal RF
74
+ - **tRF_lag**: Lag of the main peak in the temporal RF in seconds
README.md CHANGED
@@ -55,10 +55,12 @@ For NWB documentation on accessing standard containers consult https://pynwb.rea
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56
  ## 3. Reproducing the Original Analysis Table
57
 
 
 
58
  To reproduce the original table that was used to produce visualisations and anlyses in the dataset pre-print, run:
59
 
60
  ```bash
61
- python export_nwb/read_nwb_table.py /path/to/target \
62
  --filter-to-match-original \
63
  --output reconstructed_tables # Optional, saves extracted tables in .pkl and .parquet for later access
64
  ```
@@ -72,7 +74,7 @@ import os
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73
  import pandas as pd
74
 
75
- from all_gcl.export_nwb.utils.data_io import restore_numpy_arrays
76
 
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  dataset_path = "/path/to/data/storage"
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55
 
56
  ## 3. Reproducing the Original Analysis Table
57
 
58
+ We offer companion code to reproduce the analysis in our manuscript at https://github.com/eulerlab/all-GCL-manuscript .
59
+
60
  To reproduce the original table that was used to produce visualisations and anlyses in the dataset pre-print, run:
61
 
62
  ```bash
63
+ python all_gcl_manuscript/read_nwb_table.py /path/to/target \
64
  --filter-to-match-original \
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  --output reconstructed_tables # Optional, saves extracted tables in .pkl and .parquet for later access
66
  ```
 
74
 
75
  import pandas as pd
76
 
77
+ from all_gcl_manuscript.utils import restore_numpy_arrays
78
 
79
  dataset_path = "/path/to/data/storage"
80
 
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